Define these terms: substrate, enzyme, ATP, and describe why they are important in cellular respiration.In the following scenario, you will play the role of a medical examiner and analyze a case study based on scientific data.

CELL CHEMISTRY
Assignment Overview
In the following scenario, you will play the role of a medical examiner and analyze a case study based on scientific data.
You’re working at the medical examiner’s office at Pima County Hospital. It has been a slow day, with only one homicide and a dead squirrel that you checked for rabies. The squirrel was cleared for rabies and you get ready to head home. Just as you’re ready to leave, you get a call from the front desk. “We’ve got a kid you’ll want to look at right away, it looks suspicious.”
Performing autopsies on kids is your least favorite part of your job, but you realize you are needed to solve medical mysteries, and it looks like you’ve got one here.
You receive the hospital report and take note of the child’s last hours:
At 9 AM, the child’s mother returns from the store to find the little girl vomiting, not feeling well, and sleepy. The mother put her down to nap. Ten minutes later, she noticed that the child’s breathing became irregular and slow. She tried to wake her daughter, but was not able to do so. The child became comatose. At noon, the child was admitted to the hospital, with no heartbeat or spontaneous breathing.
A police report states the following:
The mother later noticed that a few of her anti-seizure pills were missing. The label indicates that the main ingredient is Amytal Sodium.
Autopsy Report
The child died within two hours of first vomiting.
Immediate cause of death was hypoxia (lack of oxygen).
Tissue sections from the kidneys, lungs, thymus, and heart show massive cell death.
Staining with cellular dyes indicates that the mitochondria within the affected tissues were damaged.
Case Assignment
For your Case Assignment, discuss your findings in a report based on the following questions:
Define these terms: substrate, enzyme, ATP, and describe why they are important in cellular respiration.
What do these components do, generally speaking, in cellular respiration? Would the reaction occur if they were not present? Why or why not?
Based on the autopsy and police reports and recalling your knowledge of cellular organelles, what general functions of the cell did the medication probably affect?
Which specific steps in the process of cellular respiration were likely affected by the medication?
Using a new chromatographic technology, you are able to determine the levels of various subcellular components in the heart cells of the child. The results are presented below:

Metabolite Autopsy Finding Normal Levels
Glucose 105 μmol 100 μmol
Pyruvate 20 μmol 25 μmol
NAD+ 10 μmol 75 μmol
NADH 350 μmol 50 μmol


 

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